RESUMO
Objective: To disrupt spa7074, which encodes a member of the TetR family transcriptional factors, in biocontrol strain Act12 and characterize the secondary metabolites in the mutant strain. Methods: We disrupted the gene spa7074 by homologous recombination. The secondary metabolites of the mutant strain Δspa7074 and Act12 were detected by HPLC. The structure was analyzed by MS and NMR. Results: Compared to the wild-type strain, the production of some unknown compounds in the mutant strain Δspa7074 increased obviously. We purified one of the compounds and identified as oligomycin D by MS and NMR analysis. Conclusion: An oligomycin D-producing strain Δspa7074 was derived via genetic engineering.
Assuntos
Metabolismo Secundário , Streptomyces/genética , Streptomyces/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Recombinação Homóloga , Espectrometria de Massas , Mutação , Rutamicina/química , Rutamicina/isolamento & purificação , Rutamicina/metabolismo , Streptomyces/química , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismoRESUMO
Quercetin (3,3',4',5,7-pentahydroxyflavone) shares certain properties with the mitochondrial ATPase inhibitor protein. At low concentrations it inhibits both soluble and particulate mitochondrial ATPase and has no effect on oxidative phosphorylation in submitochondrial particles. Unlike the mitochondrial inhibitor protein quercetin inhibits the ATP-dependent reduction of NAD+ by succinate in fully reconstituted submitochondrial particles. A comparison of various flavones indicates that the hydroxyl groups at the 3' and perhaps 3 position are important for the inhibition of ATPase activity.
